Composite

Part:BBa_K1813065

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-09-11)

LacIR pelB AgCPR ptac pelB CYP6CM1

With the A. gambiae NADPH p450 reductase ( BBa_K1813010) regulated by the inducible LacI promoter and the CYP6M1vQ ( BBa_K1813004) enzyme under constitutive regulation, this part allows one to simultaneously gauge the effectiveness of the reductase and the CYP p450, localized to the bacterial periplasm, while remaining a viable method of expression control.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BamHI site found at 1953
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1468
    Illegal NgoMIV site found at 3775
    Illegal AgeI site found at 1751
    Illegal AgeI site found at 2933
    Illegal AgeI site found at 3089
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3449


[edit]
Categories
Parameters
None