Coding

Part:BBa_K1813055

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-09-11)

pelB CYP2D6

Coding sequence of CYP2D6 ( BBa_K1813005) prefixed by the pelB localization signal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

The pelB localization signal targets a protein it is bound to for transport to the bacterial periplasm, upon which it is cleaved off. This action can bolster the stability of some proteins, due to the relatively stronger reducing conditions prevalent in the bacterial periplasm.
This part is used in BBa_K1813056

[edit]
Categories
Parameters
n/apelB CYP2D6