Coding

Part:BBa_K1813046

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-09-11)

pelB and CYP6G1

Coding sequence of CYP6G1 ( BBa_K1813004) prefiexed by the pelB localization signal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 1000
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1009


Background

The pelB localization signal targets a protein it is bound to for transport to the bacterial periplasm, upon which it is cleaved off. This action can bolster the stability of some proteins, due to the relatively stronger reducing conditions prevalent in the bacterial periplasm.
This part is used in BBa_K1813050

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Categories
Parameters
None