Status: 500 Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /websites/parts.igem.org/cgi/lib/BBDB.pm line 35.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
Compilation failed in require at /websites/parts.igem.org/cgi/extensions/wiki_header_hook.cgi line 9.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/extensions/wiki_header_hook.cgi line 9.

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LacIR, AgCPR and CYP2D6 Regulon

A construct used to achieve the simultaneous bacterial expression of CYP2D6 (BBa_K1813005) and the A. gambiae NADPH P450 reductase (BBa_K1813010) under regulative authority of the LacI repressor.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BglII site found at 3978
    Illegal BamHI site found at 1887
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4568
    Illegal AgeI site found at 1685
    Illegal AgeI site found at 2867
    Illegal AgeI site found at 3023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3383


Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of either gene without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host. In doing so, the cytochrome p450 enzyme receives a steady supply of electrons from the reductase, critical for P450 mediated catalysis, once the construct is expressed.

Status: 500 Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /websites/parts.igem.org/cgi/lib/BBDB.pm line 35.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/extensions/part_box.cgi line 9.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/extensions/part_box.cgi line 9.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.