Composite

Part:BBa_K1813024

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-08-05)

CYP2D6 Expression Cassette with LacI Reversed

A construct used to achieve the bacterial expression of CYP2D6 (BBa_K1813005) under regulative authority of the LacI repressor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BglII site found at 1737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2327
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of CYP2D6 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.

[edit]
Categories
Parameters
n/aLacI Reversed CYP2D6