Part:BBa_K1813010

A. gamb NADPH Reductase

The Anopheles gambiae NADPH - p450 reductase is a redox enzyme critical to the reuse of cytochrome p450 enzymes dependent on a supply of electrons for function.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 473
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 271
  Illegal AgeI site found at 1453
  Illegal AgeI site found at 1609
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1969
  

Background

Transferring electrons from NADPH to a cytochrome p450 enzyme, this enzyme replenishes electrons used by a cytochrome p450 in the catalysis of a reaction ensuring the reusability of the enzyme [1]. This coding sequence was synthesized and is codon optimized for E. coli. It was originally identified in Anopheles gambiae, the Anopheles Mosquito [2]. This part is also used in BBa_K1813018.

References

[1] Karunker, I., Morou, E., Nikou, D., Nauen, R., Sertchook, R., Stevenson, B. J., ... & Vontas, J. (2009). Structural model and functional characterization of the Bemisia tabaci CYP6CM1vQ, a cytochrome P450 associated with high levels of imidacloprid resistance. Insect biochemistry and molecular biology, 39(10), 697-706.