Composite

Part:BBa_K1811888

Designed by: Samuel Keating   Group: iGEM15_UMBC-Maryland   (2015-08-26)

codon optimized CUP1 copper resistance gene with RBS and promoter

Metallothionein is a copper binding protein native to S. cerevisiae that gives cells resistance to copper toxicity by binding 11 copper atoms. It prevents copper from reacting to form toxic molecules when bound and detoxifies free radicals. The part contains the metallothionein gene CUP1 (BBa_K1811222), a strong constituitive promoter (BBa_J23100), and an RBS (BBa_B0034). The CUP1 gene used in this part was codon optimized for E. coli tRNA abundance.
800px-UMBCRet2OD.jpg
UMBCRet2Cu.jpg
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None