Composite

Part:BBa_K1811666

Designed by: Samuel Keating   Group: iGEM15_UMBC-Maryland   (2015-08-26)

CUP1 metallothionein fused to LamB with promoter and RBS

LamB is a membrane channel that transports maltose and maltodextrins as well as the receptor for phage lambda. Metallothionein is a protein that sequesters 11 copper atoms and detoxifies radicals produced by copper. This part is a composite of the CUP1 LamB fusion (BBa_K1811333), a strong constituitive promoter (BBa_J23100), and an RBS (BBa_B0034).
800px-UMBCRet3OD.jpg
UMBCRet3Cu.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 212
    Illegal AgeI site found at 1460
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None