Coding

Part:BBa_K1811333

Designed by: Samuel Keating   Group: iGEM15_UMBC-Maryland   (2015-08-26)


CUP1 metallothionein fused to LamB on outer membrane

LamB is a membrane channel that transports maltose and maltodextrins as well as the receptor for phage lambda. Metallothionein is a protein that sequesters 11 copper atoms and detoxifies radicals produced by copper. This part contains two genes fused together that allows for the expression of metallothionein on the outer surface of E. coli. CUP1, the gene coding for metallothionein from S. cerevisiae, is inserted into amino acid position 153 of the LamB gene sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
    Illegal AgeI site found at 1399
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None