Device

Part:BBa_K1808011

Designed by: Remsha Afzal   Group: iGEM15_TrinityCollegeDublin   (2015-09-16)


ALDH1 regulated by the TAC promoter

This is a device that is an ALDH1 generator in a pSB1C3 plasmid backbone. It expressed ALDH1 if induced by IPTG and not repressed by tryptophan. This construct is an element of TCD iGEM 2015 for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The ALDH1 gene encodes the enzyme aldehyde dehydrogenase 1, which catalyses the conversion of (11R)-dihydroartemisinic aldehyde to dihydroartemisinic acid as well as the conversion of artemisinic aldehyde to artemisinic acid with NAD+ as the substrate. Artemisinic acid and dihydroartemisinic acid can be extracted from E. coli cells through lysis and chemically converted to the anti-malarial artemisinin. This construct was generated via 3A assembly and is optimised for expression in E. coli cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 109
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1358


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Categories
Parameters
None