Coding

Part:BBa_K1808008

Designed by: Remsha Afzal   Group: iGEM15_TrinityCollegeDublin   (2015-09-16)


Amorpha-4,11-diene synthase His tagged

This is the coding region of the ADS gene from Artemisia annua with added biobrick suffix and prefix in a pSB1C3 plasmid backbone and a 6 his tagged component. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. This particular part codes for Amorpha-4-11-diene synthase, which is an enzyme that catalyses the first step in the synthesis of antimalarial drug artemisinin by converting farnesyl diphosphate into amorpha-4,11-diene, which undergoes multiple steps to become dihydroartemisinic acid and finally artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1503
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 935
    Illegal XhoI site found at 685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1195
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None