Part:BBa_K1808007
CYP71AV1 from Artemisia annua
This is the coding region of the CYP71AV1 gene from Artemisia annua with added biobrick suffix, prefix and a 6 His tag in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The CYP71AV1 gene encodes the enzyme Amorphadiene oxidase, which catalyses the conversion of amorpha-4,11-diene to artemisinic alcohol, subsequent conversion of the alcohol to artemisinic aldehyde and the subsequent and final conversion of the artemisinic aldehyde to artemisinic acid with NADP+ as the substrate. Artemisinic acid and dihydroartemisinic acid can be extracted from E. coli cells through lysis and chemically converted to the anti-malarial artemisinin. The Amorphadiene oxidase enzyme is a memberofthe cytochrome p450 family of hemoproteins and is the rate limiting enzyme in the synthesis pathway for artemisinic acid developed by Amyris. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 515
- 1000COMPATIBLE WITH RFC[1000]
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