Coding

Part:BBa_K1808006

Designed by: Remsha Afzal   Group: iGEM15_TrinityCollegeDublin   (2015-09-16)


CYB5 HIS from Artemisia annua

This is the coding region of the CYB5 HIS gene from Artemisia annua with added biobrick suffix and prefix in a pSB1C3 plasmid backbone and a 6 HIS TAG. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The CYB5 was used by our team to increase the efficiency of the rate limiting step of Cytochrome P450 which catalyses the conversion of amorphadiene to artemisinic alcohol to artemisinic aldehyde and finally to artemisinic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None