Part:BBa_K1808000
DBR2 gene from Artemisia annua
This is the coding region of the DBR2 gene from Artemisia annua with added biobrick suffix and prefix in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The DBR2 gene encodes the enzyme artemisinic aldehyde delta11(13)-reductase, which catalyses the conversion of artemisinic aldehyde to (11R)-dihydroartemisinic aldehyde with NADPH as the electron donor substrate. The product can be acted upon by the Artemisia annua aldehyde dehydrogenase 1 enzyme (see part BBa_K1808001) to give rise to dihydroartemisinic acid, which can be isolated and converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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