Polyphosphate kinase enzyme from Escherichia coli
Coding sequence for the Polyphosphate enzyme (PPK) from Escherichia coli.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 317
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 1671
- 1000COMPATIBLE WITH RFC
iGEM2019_Nanjing China Experiment
This year our team develops a simple solo medium-copy plasmid-based polyphosphate kinase (PPK1) overexpression strategy for achieving maximum intracellular polyphosphate accumulation, so the data can provide some reference to this part.
We test supernatant Pi concentration and optical density of Ecoil(DH5a) and DH5a-MDPP in Synthetic municipal wastewater(SMW).
Ps: SMW means Synthetic municipal wastewater
DH5a-MDPP means solo medium-copy DH5a ppk in DH5a