Composite

Part:BBa_K180012

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-19)

Rock - Primary plasmid (part 2) [tac pR, mCherry and LasI generator]

Part of the iGEM2009 Team:Southampton rock-paper-scissors 'rock' primary plasmid.

mCherry and LasI are generated under tac pR regulation.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1825
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1825
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1825
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1825
    Illegal AgeI site found at 2430
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/bidirectional
//function/reporter/fluorescence
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controltrp, lac
directionforward
emission610 nm
emit610
excitation587 nm
excite587
lum
n/aRock - Primary plasmid (part 2) [tac pR, mCherry and LasI generator]
proteinmCherry