Composite

Part:BBa_K180011

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-19)

Rock - Primary plasmid (part 1) [RhlR generator]

Part of the iGEM2009 Team:Southampton rock-paper-scissors 'rock' primary plasmid.

RhlR is constitutively produced under promotion by promoter J23119.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/bidirectional
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controlconstitutive
device_typerhlR generator
directionforward
n/aGoL - Primary plasmid (part 1)/RPS - Paper primary plasmid (part 1) [LuxR generator]
proteinrhlR