Composite

Part:BBa_K1797008

Designed by: iGEM15_Tsinghua   Group: iGEM15_Tsinghua   (2015-09-17)

PCB generation system

Fig.1 PCB generation pathway (source: Ken Okada, HO1 and PcyA proteins involved in phycobilin biosynthesis form a 1:2 complex with ferredoxin-1 required for photosynthesis, FEBS Letters, Volume 583, Issue 8, 17 April 2009, Pages 1251-1256, ISSN 0014-5793)

This part includes ho1 and PcyA, both of which are needed in converting heme into PCB(phycocyanobilin). PCB is an important part of the Cph1, a red light sensor. Since PCB cannot be generated naturally by E.coli. This part can be used to produce PCB and make sure the E.coli can sense red light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1564
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1117
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None