Composite

Part:BBa_K1797003

Designed by: iGEM15_Tsinghua   Group: iGEM15_Tsinghua   (2015-09-17)

Blue Light Sensing System with GFP and RFP Reporter

This part is one of the many bio-bricks used to test the blue-light system in our project. Specifically, it contains an entire blue-light system, including a light sensor YF1 and a response regulator FixJ, and two Fixk2 promoter regulated reporters. We used this part to test the efficacy and efficiency of the blue-light system in E. coli. If the bacteria carrying this plasmid is provided with blue light, two reporters cannot be synthesized. On the contrary, if it is kept in dark, then the bacteria colony will display a mixture color from RFP and GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1338
    Illegal NgoMIV site found at 1410
    Illegal NgoMIV site found at 1500
    Illegal NgoMIV site found at 1518
    Illegal NgoMIV site found at 2030
    Illegal NgoMIV site found at 2323
    Illegal NgoMIV site found at 2417
    Illegal AgeI site found at 617
    Illegal AgeI site found at 729
    Illegal AgeI site found at 1052
    Illegal AgeI site found at 2198
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2087
    Illegal BsaI.rc site found at 951


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Categories
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