Regulatory

Part:BBa_K1796201

Designed by: Yu Luo   Group: iGEM15_SCU_China   (2015-09-16)


An unloaded sgRNA that contains BbsI cutting site, with a promoter and terminator.

This part contains a sgRNA which we designed to be expressed in procaryotic organism. There is a promoter in fron of it and a terminator behind.Instead of a certain crRNA, we designed a BbsI cutting site in the middle of the sgRNA, on the place where the crRNA should be. So we can use this part to knockout any sequence as we want in procaryotic organism by the way of CRISPR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None