Part:BBa_K1796104

Corrinoid Iron-Sulfur Protein

The CFeSP is the key of CODH/ACS. It's an intermediate carrier of methyl. The CFeSP must have a highly conserved function as part of the Wood-Ljungdahl pathway, since homologs of the CFeSP genes are not found in any genome that does not also have acetyl-CoA synthase and other Wood-Ljungdahl pathway genes.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 999
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 999
  Illegal NheI site found at 862
  Illegal NotI site found at 163
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 999
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 999
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 999
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 709
  

Parameter of Protein

Number of amino acids: 446

Molecular weight: 48153.2

Theoretical pI: 5.38

Amino acid composition: 

Ala (A) 65 14.6%

Arg (R) 19 4.3%

Asn (N) 19 4.3%

Asp (D) 26 5.8%

Cys (C) 5 1.1%

Gln (Q) 10 2.2%

Glu (E) 31 7.0%

Gly (G) 25 5.6%

His (H) 7 1.6%

Ile (I) 24 5.4%

Leu (L) 49 11.0%

Lys (K) 28 6.3%

Met (M) 6 1.3%

Phe (F) 11 2.5%

Pro (P) 21 4.7%

Ser (S) 21 4.7%

Thr (T) 24 5.4%

Trp (W) 2 0.4%

Tyr (Y) 16 3.6%

Val (V) 37 8.3%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 57

Total number of positively charged residues (Arg + Lys): 47

Atomic composition:Carbon C 2151

Hydrogen H 3454

Nitrogen N 576

Oxygen O 651

Sulfur S 11

Formula: C2151H3454N576O651S11Total number of atoms: 6843

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 35090

Abs 0.1% (=1 g/l) 0.729, assuming all pairs of Cys residues form cystines

Ext. coefficient 34840

Abs 0.1% (=1 g/l) 0.724, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 25.50

This classifies the protein as stable.

Aliphatic index: 102.47

Grand average of hydropathicity (GRAVY): 0.008