Part:BBa_K1796013
hesA promoted from Paenibacillus sp. WLY78
Function has not so far been determined , but Deletion analysis indicates that in addition to the core nif genes, hesA plays an important role in nitrogen fixation and is responsive to the availability of molybdenum. HesA a member of the ThiF-MoeB-HesA family and contains an N-terminal nucleotide binding domain and a C-terminal MoeZ/MoeB-like domain. The ThiF-MoeB-HesA family engage in an ATP-dependent process that activates the C-terminus of partner ubiquitin-like proteins by forming an acyl adenylate complex that facilitates sulfur transfer.
First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline. Sequence submit is promoted by the synthesis company, containing a PstI in the gene
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 143
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 143
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 143
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 143
- 1000COMPATIBLE WITH RFC[1000]
Parameter of Protein
Number of amino acids: 253
Molecular weight: 27608.7
Theoretical pI: 5.29
Amino acid composition:
Ala (A) 25 9.9%
Arg (R) 17 6.7%
Asn (N) 7 2.8%
Asp (D) 12 4.7%
Cys (C) 8 3.2%
Gln (Q) 7 2.8%
Glu (E) 20 7.9%
Gly (G) 27 10.7%
His (H) 7 2.8%
Ile (I) 13 5.1%
Leu (L) 27 10.7%
Lys (K) 6 2.4%
Met (M) 12 4.7%
Phe (F) 3 1.2%
Pro (P) 12 4.7%
Ser (S) 10 4.0%
Thr (T) 12 4.7%
Trp (W) 3 1.2%
Tyr (Y) 9 3.6%
Val (V) 16 6.3%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:Carbon C 1203
Hydrogen H 1919
Nitrogen N 341
Oxygen O 363
Sulfur S 20
Formula: C1203H1919N341O363S20Total number of atoms: 3846
Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 30410
Abs 0.1% (=1 g/l) 1.101, assuming all pairs of Cys residues form cystines
Ext. coefficient 29910
Abs 0.1% (=1 g/l) 1.083, assuming all Cys residues are reduced
Estimated half-life:The N-terminal of the sequence considered is V (Val).
The estimated half-life is: 100 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:The instability index (II) is computed to be 44.33
This classifies the protein as unstable.
Aliphatic index: 89.88
Grand average of hydropathicity (GRAVY): -0.077
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