Composite

Part:BBa_K1792004

Designed by: Brian Dempsey   Group: iGEM15_Lethbridge_HS   (2015-09-17)


Nuclease - secreted with Cterm 6xHis tag

Nuclease from Staphylococcus aureus. This nuclease is secreted due to a bacterial N-terminal export tag. The nuclease contains a C-terminal 6xHis tag for possible purification and concentration. This part is the same as BBa_K729004 with the addition of the his-tag. Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides (Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.)

The protein is undercontrol of an IPTG inducible promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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