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Part:BBa_K1792003

Designed by: Brian Dempsey   Group: iGEM15_Lethbridge_HS   (2015-09-17)


alpha-Dextranase, secreted - Basic part

This part exists as a Composite part only. See BBa_1792002.

alpha-Dextranase from Chaetomium gracile (Fungi). Performs catalysis of the endohydrolysis of 1,6-alpha-D-glucosidic linkages in dextran. This is a very large gene (1,818 nucleotides, native sequence). The Nterminal 14 amino acids are not included due to disorder. An bacterial Nterm export tag is included in the sequence. This protein has a Cterminal 6XHis tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1662
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 358


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