Intermediate

Part:BBa_K179004

Designed by: Hiroyuki Yamazaki   Group: iGEM09_Todai-Tokyo   (2009-10-18)


Yeast Gal1 promoter + LDLR

Constitutive yeast Gal1 promoter and LDLR ORF

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1273
    Illegal PstI site found at 617
    Illegal PstI site found at 1644
    Illegal PstI site found at 1908
    Illegal PstI site found at 2705
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1273
    Illegal PstI site found at 617
    Illegal PstI site found at 1644
    Illegal PstI site found at 1908
    Illegal PstI site found at 2705
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1273
    Illegal BglII site found at 2259
    Illegal BamHI site found at 1632
    Illegal BamHI site found at 2003
    Illegal BamHI site found at 2127
    Illegal XhoI site found at 3099
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1273
    Illegal PstI site found at 617
    Illegal PstI site found at 1644
    Illegal PstI site found at 1908
    Illegal PstI site found at 2705
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1273
    Illegal PstI site found at 617
    Illegal PstI site found at 1644
    Illegal PstI site found at 1908
    Illegal PstI site found at 2705
    Illegal NgoMIV site found at 1310
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None