Coding

Part:BBa_K179001

Designed by: Koji Tanaka   Group: iGEM09_Todai-Tokyo   (2009-10-18)

leucine dehydrogenase

Leucine dehydrogenase from Bacillus subtilis[http://www.uniprot.org/uniprot/?query=YqiT&sort=score]. This protein makes leucine into isovaleric acid, which make unpleasant odor when expressed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 422


Functional Parameters

We measured the intensity of smell of cleansed air, air, air in the test tube in which we were shaking culture of E.coli “negative control”, and air in the test tube in which we were shaking culture of E.coli which has plasmid containing “promoter + RBS + yqiT + double terminator”

Smell.png

Data
Time for shake culture (each of sample) 15 hours
Negative control has plasmid containing “yqiT + double terminator” (Not including promoter)
Cleansed air is cleansed with active carbon filter.
Equipment

  • Trade name: ODOR CONCENTRATION METER
  • Model: XP-329
  • Serial No. 311059
  • Manuf. NEW COSMOS ELECTRIC CO,LTD.
  • Setting: Peak hold



[edit]
Categories
Parameters
None