Composite

Part:BBa_K1780018

Designed by: Prachiti Moghe   Group: iGEM15_IISER_Pune   (2015-09-18)


Bacterial population growth control device

LuxI gene under the control of an inducible promoter, produces AHL. At high concentration of AHL, LuxR-AHL complex binds to promoter lux pR, leading to increased expression of CcdB, which triggers cell death.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
    Illegal BamHI site found at 2622
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1873
    Illegal BsaI.rc site found at 2072


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