Composite

Part:BBa_K1780017

Designed by: Prachiti Moghe   Group: iGEM15_IISER_Pune   (2015-09-18)


Killer Gene, induced by AHL

When there is AHL present in the medium, it forms an activated complex with LuxR, which enhances the transcription of genes downstream of promoter lux pR. Thus, cell death is triggered at high concentration of AHL, due to expression of CcdB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1753
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 1203


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