Coding

Part:BBa_K1779214

Designed by: Tao Li   Group: iGEM15_CHINA_CD_UESTC   (2015-09-09)

mamAB Operon 10

The mamAB Operon is sufficient for magnetite biomineralization. As the length of the mamAB operon is about 17kb, we divided this operon into 10 parts. This part is known as "Part ten" of the full 17kb mamAB operon. It contains the rest of mamU gene and the hypothesis termination of the operon. This part can also be assembled with other parts by Gibson assemble and restriction endonuclease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 620
    Illegal BamHI site found at 220
    Illegal XhoI site found at 616
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 597
    Illegal AgeI site found at 255
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None