Coding

Part:BBa_K1779211

Designed by: Tao Li   Group: iGEM15_CHINA_CD_UESTC   (2015-09-09)

mamAB Operon 07

The mamAB Operon is sufficient for magnetite biomineralization. As the length of the mamAB operon is about 17kb, we divided this operon into 10 parts. This part is known as "Part seven" of the full 17kb mamAB operon. It contains the rest of the mamO, the mamP gene, the mamA gene and part of the mamQ gene. So you can use it as template for the mamP and mamA gene, and this part can also be assembled with other parts by Gibson assemble and restriction endonuclease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1340
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2221
    Illegal NgoMIV site found at 2305
    Illegal AgeI site found at 85
    Illegal AgeI site found at 767
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1805


[edit]
Categories
Parameters
None