Coding

Part:BBa_K1779210

Designed by: Tao Li   Group: iGEM15_CHINA_CD_UESTC   (2015-09-09)

mamAB Operon 06

The mamAB Operon is sufficient for magnetite biomineralization. As the length of the mamAB operon is about 17kb, we divided this operon into 10 parts. This part is known as "Part six" of the full 17kb mamAB operon. It contains the part of the mamN O. This part can be assembled with other parts by Gibson assemble and restriction endonuclease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1403
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 289
    Illegal AgeI site found at 1268
    Illegal AgeI site found at 1352
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1883
    Illegal BsaI.rc site found at 554


[edit]
Categories
Parameters
None