Coding

Part:BBa_K1779209

Designed by: Tao Li   Group: iGEM15_CHINA_CD_UESTC   (2015-09-09)

mamAB Operon 05

The mamAB Operon is sufficient for magnetite biomineralization. As the length of the mamAB operon is about 17kb, we divided this operon into 10 parts. This part is known as "Part five" of the full 17kb mamAB operon. It contains the part of the mamM N gene. This part can be assembled with other parts by Gibson assemble and restriction endonuclease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1254
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1492
    Illegal BsaI.rc site found at 325
    Illegal BsaI.rc site found at 886


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Parameters
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