Coding

Part:BBa_K1779208

Designed by: Tao Li   Group: iGEM15_CHINA_CD_UESTC   (2015-09-09)

mamAB Operon 04

The mamAB Operon is sufficient for magnetite biomineralization. As the length of the mamAB operon is about 17kb, we divided this operon into 10 parts. This part is known as "Part four" of the full 17kb mamAB operon. It contains the rest of mamK gene，the mamL gene and part of the mamM. So you can use it as template for the mamL gene, and this part can also be assembled with other parts by Gibson assemble and restriction endonuclease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 125
    Illegal NgoMIV site found at 272
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 800


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Parameters
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