Coding

Part:BBa_K1779207

Designed by: Tao Li   Group: iGEM15_CHINA_CD_UESTC   (2015-09-09)

mamAB Operon 03

The mamAB Operon is sufficient for magnetite biomineralization. As the length of the mamAB operon is about 17kb, we divided this operon into 10 parts. This part is known as "Part three" of the full 17kb mamAB operon. It contains the part of the mamE, mamJ gene and part of the mamK. So you can use it as template for the mamJ gene, and this part can also be assembled with other parts by Gibson assemble and restriction endonuclease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 805
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 713
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 196
    Illegal NgoMIV site found at 1560
    Illegal NgoMIV site found at 2446
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2058


[edit]
Categories
Parameters
None