Coding

Part:BBa_K1779101

Designed by: Guang Zhang   Group: iGEM15_CHINA_CD_UESTC   (2015-09-04)

Magnetosome mamXY operon

The mamXY operon encodes proteins related to the magnetosome membrane (mamY, X, Z, and ftsZ-like genes) and its deletion causes Magnetospirillum cells to produce smaller magnetite particles with superparamagnetic characteristics. You can degist the recombinant plasmid with the restriction enzyme (EcoRI, XbaI, SpeI and PstI) to get the fragments of this operon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1427
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 474
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1708
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3551
    Illegal SapI.rc site found at 2275


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Categories
Parameters
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