Coding

Part:BBa_K1779100

Designed by: Guang Zhang   Group: iGEM15_CHINA_CD_UESTC   (2015-09-04)

Magnetosome mamCD operon and mms6 operon

The mamCD operon (composed of the genes mamC, D, F, and G) and the mms6 operon (contains mms6, mmsF, mgr4070, mgr4071, and mgr4074) are involved in magnetite crystal shape and size. The deletion of both the mamCD and mms6 operons can seriously affect both the morphology of the magnetite magnetosome crystals and the alignment of the magnetosomes. You can amplify the sequence derictly with PCR method if you want to put magnetosome in your project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2533
    Illegal NheI site found at 3674
    Illegal NheI site found at 4507
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 335
    Illegal BamHI site found at 5584
    Illegal XhoI site found at 602
    Illegal XhoI site found at 1484
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3226
    Illegal NgoMIV site found at 4271
    Illegal NgoMIV site found at 6030
    Illegal NgoMIV site found at 6120
    Illegal AgeI site found at 5203
    Illegal AgeI site found at 5455
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 639
    Illegal BsaI site found at 4237
    Illegal BsaI site found at 4707
    Illegal BsaI site found at 4974
    Illegal BsaI site found at 6095
    Illegal BsaI.rc site found at 2756
    Illegal SapI site found at 3094
    Illegal SapI site found at 4656
    Illegal SapI site found at 5809


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Categories
Parameters
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