DNA

Part:BBa_K1778006

Designed by: Lehua Jia   Group: iGEM15_Scut-Champion-Park   (2015-09-11)

Kana-His is a selective marker gene in experiment.

Kana-His is a selective marker gene in experiment. The escherichia coli with Kana-His gene has the resistance of kanamycin. The pichia pastoris with Kana-His gene can grow in the culture medium without histidine. It can help to screen the transformant.

In the plasmid pPIC9K, this part play the role of selection marker. We use the part as the selection marker. We use the culture medium with antibiotic or the culture medium without histidine to screen.We buy the plasmid pPIC9K and get the segment using PCR.We will improve this part to reduce the use of antibiotic gene and purify the bacterial strain by auxotroph mutant recovery method.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 446
    Illegal PstI site found at 3038
    Illegal PstI site found at 4279
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 207
    Illegal NheI site found at 1154
    Illegal PstI site found at 3038
    Illegal PstI site found at 4279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 4123
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 446
    Illegal PstI site found at 3038
    Illegal PstI site found at 4279
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 446
    Illegal PstI site found at 3038
    Illegal PstI site found at 4279
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2494
    Illegal BsaI site found at 4595


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Categories
Parameters
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