Composite

Part:BBa_K1778002

Designed by: Lehua Jia   Group: iGEM15_Scut-Champion-Park   (2015-09-11)

TRE-CYC1TATA is a recombinant promoter, which is constructed in order to make the Tet-on system func

TRE-CYC1TATA is a recombinant promoter, which is constructed in order to make the Tet-on system function in Pichia pastoris, which is connected in series by TRE promoter and CYC1TATA sequence. The recombinant promoter can be combined with the correct conformation of rtTA to form a regulation mechanism to ensure the validity of the switch.

TRE-CYC1TATA consists of two parts of the TRE promoter and CYC1TATA sequence. The two sub sections have been described in their respective natural functions.Using the part as a touch switch of recombinant yeast cell to the external environment of tetracycline analogues,make sure that it is able to play a role in the host cell.TRE-CYC1TATA consists of two parts of the TRE promoter and CYC1TATA sequence, which is connected in series by the PCR technology.We will have the opportunity to do further optimization to make it more suitable for the host cells of Pichia pastoris, which can improve the sensitivity of the detector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None