RNA

Part:BBa_K1777001

Designed by: Xilin Jiang   Group: iGEM15_Fudan   (2015-09-13)


beta-globin intron1 SA with 3 mir-21 targeting sites

This part has a beta-globin intron1 SA and 3 mir-21 binding domain. This part can be used to build a 6-binding-site mir-21 sponge with another part(BBa_K1777002) with a beta-globin intron 1 SB. This part has a overlap region which overlaps the part(BBa_K1777002), and overlap PCR can be used to connect these two parts.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 114
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 114
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 114
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 114
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 114
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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