Inverter

Part:BBa_K1774003

Designed by: HKU iGEM 2015   Group: iGEM15_Hong_Kong_HKU   (2015-09-18)

trpR-pTrp inverter (RBS-trpR-TT-pTrp)

Overview

This is a switch composed of 2 basic parts, trpR, E.coli tryptophan repressor and ptrp, tryptophan promoter and operator. Under the presence of sufficient amount of tryptophan the switch remains silent, however when the environment lacks tryptophan, the expression of the switch in is on. This can be used as a killing switch in synthetic organisms to prevent the escape of bacteria.

Addition of Trp repress anything downstream of the inverter. Notice that it contain illegal SpeI sites, however it is essential for the functioning of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 513
    Illegal SpeI site found at 521
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 513
    Illegal SpeI site found at 521
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 513
    Illegal SpeI site found at 521
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 513
    Illegal SpeI site found at 521
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 193


[edit]
Categories
Parameters
None