Generator

Part:BBa_K1773020

Designed by: Sarunas Tumas   Group: iGEM15_Vilnius-Lithuania   (2015-08-26)

Cas3 gene with pLux/cI right promoter and Medium RBS

Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is a helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and medium RBS site. We wanted to have different expression strengths of Cas3, so we cloned it also to a strong RBS (BBa_K1773019), and a weak RBS (BBa_K1773021).

In the I-F type CRISPR-Cas system Cascade complex (BBa_K1773005) distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2720
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1441
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2404
    Illegal AgeI site found at 2777
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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