Generator

Part:BBa_K1773019

Designed by: Sarunas Tumas   Group: iGEM15_Vilnius-Lithuania   (2015-08-26)

Cas3 gene with pLux/cI right promoter and strong RBS

Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is a helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and strong RBS site. We wanted to have different expression strengths of Cas3, so we cloned it also to a Medium RBS (BBa_K1773020), and a weak RBS (BBa_K1773021)

In the I-F type CRISPR-Cas system Cascade complex (BBa_K1773005) distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2722
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1443
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2406
    Illegal AgeI site found at 2779
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None