Deleted
Sample Discontinued
Experience: None
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Part:BBa_K1773013

Designed by: Sarunas Tumas   Group: iGEM15_Vilnius-Lithuania   (2015-08-23)


Lux Right promoter with strong RBS sequence and Cas3 protein

This part contains the Lux right promoter and strong RBS site attached to the I-F type CRISPR-Cas system Cas3 protein. Cas3 is a helicase-nuclease protein, which degrades single stranded DNA in a 3'-5' direction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2725
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1446
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2409
    Illegal AgeI site found at 2782
  • 1000
    COMPATIBLE WITH RFC[1000]


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