Part:BBa_K1772003

Lignin Peroxidase

Protein coding sequence of Lignin Peroxidase, an enzyme that is the initiator and one of the main enzymes responsible for the breakdown of lignin inside the cell walls of plant cells. Reticulitermes flavipes, or the eastern subterranean termite, uses this enzyme to digest woody debris with higher efficiency. Lignin peroxidase has the ability to oxidize aromatic compounds in lignin with redox potentials above 1.4 eV, which is the equivalent to reducing metals like gold or molecules such as lead oxide. It is believed that this action is done by catalyzing the transfer of an oxygen atom from a hydrogen peroxide molecule to an aromatic ring. However, the exact redox mechanism for lignin peroxidase is currently unknown.

Parameters:

pH Range: 6.8-7.2

Temperature Range: 30-37 degrees C

Sources:

Piontek, K., Smith, A., & Blodig, W. (2001, May 29). Lignin peroxidase structure and function. Retrieved September 16, 2015, from [http://www.ncbi.nlm.nih.gov/pubmed/11356137]

Sigma-Aldrich (accessed 2015, September 17). Lignin Peroxidase. [http://www.sigmaaldrich.com/catalog/product/sigma/42603?lang=en&region=US]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1453
  Illegal EcoRI site found at 1497
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1453
  Illegal EcoRI site found at 1497
  Illegal NheI site found at 1261
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1453
  Illegal EcoRI site found at 1497
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1453
  Illegal EcoRI site found at 1497
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1453
  Illegal EcoRI site found at 1497
  Illegal NgoMIV site found at 226
  
• 1000
  COMPATIBLE WITH RFC[1000]