Composite

Part:BBa_K1772000

Designed by: Tianyu Tan   Group: iGEM15_Purdue   (2015-09-17)

yEGFP-tagged Mn peroxidase

Manganese Peroxidase fusion protein gene flanked by two linker sequences, with a a GFP tag on the N-terminus and a yeast secretion tag on the C-terminus. A constitutive medium strength pCyc promoter followed by a yeast kozak sequence caps the N-terminus, and a yeast terminator sequence caps the C-terminus. Sequence optimized for Yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 346
    Illegal PstI site found at 1018
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 346
    Illegal PstI site found at 1018
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 788
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 346
    Illegal PstI site found at 1018
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 346
    Illegal PstI site found at 1018
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None