Generator

Part:BBa_K177029

Designed by: Michael Lower   Group: iGEM09_Warsaw   (2009-07-22)


Redflag.png

Safety Flag

The iGEM Safety Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety (AT) igem (DOT) org with any questions.

Reason: Listeriolysin and Invasin parts

If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2017 Safety Page for more information.


cell Invasion device (testing)

makes E. coli a good bioweapon by allowing bacteria to enter and live inside human cells

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4093
    Illegal XbaI site found at 2080
    Illegal SpeI site found at 1894
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4093
    Illegal NheI site found at 1607
    Illegal NheI site found at 2269
    Illegal SpeI site found at 1894
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4093
    Illegal BglII site found at 2077
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4093
    Illegal XbaI site found at 2080
    Illegal SpeI site found at 1894
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4093
    Illegal XbaI site found at 2080
    Illegal SpeI site found at 1894
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2065
    Illegal EcoRI site found at 4093
    Illegal XbaI site found at 2080
    Illegal SpeI site found at 1894


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Categories
Parameters
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