Device

Part:BBa_K177004

Designed by: Michael Lower   Group: iGEM09_Warsaw   (2009-02-18)


Redflag.png

Safety Flag

The iGEM Safety Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety (AT) igem (DOT) org with any questions.

Reason: Listeriolysin and Invasin parts

If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2017 Safety Page for more information.


bistable_1_R

right portion of bistable 1 switch

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5537
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1223
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2096
    Illegal NgoMIV site found at 6441
    Illegal AgeI site found at 1605
    Illegal AgeI site found at 1929
    Illegal AgeI site found at 2064
    Illegal AgeI site found at 2142
    Illegal AgeI site found at 3651
    Illegal AgeI site found at 4155
    Illegal AgeI site found at 7289
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8109


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Categories
Parameters
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