Composite

Part:BBa_K1768005

Designed by: Gert Pietersen   Group: iGEM15_Pretoria_UP   (2015-09-18)

Invertible promoter with RFP reporter

This part is for the characterisation of the Invertible Promoter part BBa_K1768001 which is a permanently inducible gene switch.

An antisense copy of a constitutive promoter flanked by Lox66 and Lox71 recombination recognised by the Cre enzyme which facilitates a single round of recombination between these sites. Thus the orientation of the promoter can be inverted, allowing expression of RFP reporter gene.

This part responds to POPS of an upstream part, BBa_K1768004, to induce a downstream activation of the RFP protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 115
    Illegal NheI site found at 138
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 844
    Illegal AgeI site found at 956
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
regulator
Parameters
None