Inverter

Part:BBa_K1768003

Designed by: Gert Pietersen   Group: iGEM15_Pretoria_UP   (2015-09-14)


Recombinase invertible constitutive promoter 2

A reverse compliment of a constitutive promoter flanked by Lox71 and Lox66 which are recognised by the Cre enzyme and facilitates a single round of recombination between these sites. Thus the direction of the promoter can be changed allowing expression of a different gene than in the initial state. This part is the same as part BBa_K1768001 but Lox71 is upstream of the promoter with a downstream lox66.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 115
    Illegal NheI site found at 138
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None