Part:BBa_K1766013
EnvZ_V5 osmoregulatory histidine kinase from E.coli.
EnvZ is part of the EnvZ-OmpR two-component regulatory system for controlling osmotic pressure in E.coli. Structure includes the EnvZ247 allele [1] which is linked through a GGSSAAG linker to a V5 epitope tag. The full structure has a predicted size of 115.36 kDa [2]. The part was provided in the pRD400 (or pEnvZ) backbone under a lac-inducible promoter [3].
Two-component regulatory systems are a common in procaryotes for signal transduction through the membrane. In this case, EnvZ is activated by changes in osmotic pressure leading to phosphorylation of the kinase. The response regulator OmpR is then activated throungh phosphorylation resultning in the regulation of expression of the outer membrane porin proteins OmpF and OmpC.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 316
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 316
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 316
Illegal BglII site found at 236 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 316
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 316
- 1000COMPATIBLE WITH RFC[1000]
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